![]() ![]() Start by bringing numbers with you: numbers around how equal pay leads to better retention and numbers about company profit (and therefore being able to afford it). It’s a very delicate thing for workers to advocate for. Generally, you should only bring this conversation up if you hold sway within the company, or you have the backing of a lot of fellow employees. If your employer doesn’t currently do annual audits (and most don’t), you might be able to advocate at work for it. A 2019 pay audit at Google found that it was underpaying men so imagine what women of color are dealing with. While this is most likely to benefit women of color at work, it’s also a good protection for all workers. Putting an annual salary review into place would allow companies to see if new hires, hires across different departments and if raises for all their employees are fair and equal. We know there is a systemic pay gap, but companies shy away from reviewing their salary data to find out what’s going on in their own businesses. A negotiation-specific training would give Latinas the personal tools they need to negotiate for more money and more work benefits. five years later, I’m still at the company and feel taken care of.”Ĭompanies that provide employee training see more employee retention, according to a LinkedIn survey from 2019. In 2018, my company paid for negotiation training for 40 of us and it changed my life for the better. “The corporate world is just one long negotiation process and giving women access to this training will help them be more effective in their jobs and when negotiating better salaries. “Paying for negotiation training would be the most cost-effective silver bullet for companies to close the wage gap and improve productivity,” Vanessa Menchaca Wachtmeister from Wander Onwards, a travel and wealth platform, tells HipLatina. Provide negotiation training for employees. Furthermore, women who take paid leave are 93 percent more likely to be in the workforce nine to 12 months after a child’s birth than women who take no leave, according to the National Partnership for Women & Families. Latinxs are the least likely to have access to paid sick days (only 38.4 percent) or paid parental leave (only 25.1 percent) of any racial or ethnic group, American Progress reported. The pay gap is even worse for Latina mothers who earn 53 percent less than white fathers, LeanIn reported. California’s program also significantly reduced food insecurity among households following childbirth”. A 2021 report from the Center on Budget and Policy Priorities found that California’s paid parental leave program “…lowered the risk of poverty among mothers of infants by 10.2 percent and increased household income for those mothers by 4.1 percent, on average. Not having something like paid parental leave means a worker must use their own pay to arrange time off to have a child. These industries are famous for their mistreatment of workers. Guess how many paid sick days a field worker is granted according to the US government? Zero. Bureau of Labor Statistics, Latinxs are most likely to have a job in fields like construction, agriculture, and hospitality.
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![]() Instead of the NodeSource repository, the AppStream's version of Node.js has to be disabled. # As yum will try to install Node.js from the AppStream repository + rpm -q -whatprovides redhat-release || rpm -q -whatprovides centos-release || rpm -q -whatprovides cloudlinux-release || rpm -q -whatprovides sl-release # Installing the NodeSource Node.js 12.x repo. Use the following command to install Node.js yum repository in your Linux server. # dnf install -y gcc-c++ makeīefore installing Node.js, we need to add Node.js official yum repository in our Linux server. Therefore, we are installing these packages by using dnf command. Node.js requires following software packages for compilation and to make the executables. Therefore, you need to install Node.js on your Linux server. Rocket Chat requires Node.js server to run its services. Installing Node.js Server on CentOS / RHEL 8: ![]() "hash" : BinData(0,"AAAAAAAAAAAAAAAAAAAAAAAAAAA="), Using a default configuration for the set", # mongo -eval "printjson(rs.initiate())"Ĭonnecting to: mongodb://127.0.0.1:27017/?compressors=disabled&gssapiServiceName=mongodb # sed -i "s/^#replication:/replication:\n replSetName: rs01/" /etc/nfĮnable and start MongoDB database service. # dnf install -y mongodb-orgĬonfigure following settings in MongoDB configuration files as required by Rocket Chat software. Now, you can easily install MongoDB server on CentOS / RHEL 8 by executing dnf command. MongoDB Repository 9.4 kB/s | 12 kB 00:01 īaseurl=$releasever/mongodb-org/4.2/x86_64/īuild cache for MongoDB yum repository. # vi /etc//mongodb-org-4.2.repoĪdd following directives in this file. To install MongoDB database server, you need to add MongoDB official yum repository in your Linux operating system.Ĭreate a repo file for MongoDB yum repository by using vim text editor. Installing MongoDB Server on CentOS / RHEL 8: # uname -rĬheck version of your Linux operating system. # dnf update -yĬheck Kernel version of your Linux operating system. Hostname – Ĭonnect with as root user by using a ssh client.īy following the best practice, you should update all the software packages in Linux operating system before installing a new software.We are using a minimally installed CentOS 8 virtual machine with following specification. Some of the major features of Rocket Chat are: It is a great solution for communities and companies wanting to privately host their own chat service or for developers looking forward to build and evolve their own chat platforms. Rocket Chat is free and open source and distributed under MIT license. Rocket Chat is a web chat server, developed in JavaScript, by using the Meteor full stack framework. Installing Rocket Chat Server on CentOS / RHEL 8.Installing GraphicsMagick on CentOS / RHEL 8. ![]()
G et started for free and upgrade your medical workflow to global standards. Furthermore, it features reliable 256-bit security and HIPAA compliance to meet the medical industry’s needs. ![]() It allows medical providers to prescribe medications digitally while maintaining a solid fax presence when needed. ![]() With iFax, you can integrate your free e-prescribing software with powerful online fax capabilities. Fax remains a valuable asset for the medical industry, and you’d do well to find an online service with forward-looking features. That said, there are other tools you need to enhance your medical practice. It has allowed for straightforward digital prescriptions, saving time and money for all participants. The Ohio Board of Pharmacy has developed a set of frequently asked questions providing additional guidance regarding the new law. 1, 2023, that all Medicare Part D plans use e-prescribing for Schedule II-V controlled substances. The introduction of free e-prescribing software has been an undeniable game changer for many healthcare institutions across the globe. The state bill goes into effect just over three months before a federal requirement beginning Jan. Try iFax to Augment Your Free E-Prescribing Software On top of that, healthcare institutions have experienced streamlined workflows and better record management thanks to electronic prescriptions. The pharmacists tasked with retrieving medications also have an easier time interpreting prescriptions, resulting in quicker services. Meanwhile, patients can receive their prescriptions online and with minimal downtime. What Are the Benefits of E-Prescription Services?ĭigital prescription benefits all parties involved, as it makes the process more convenient for doctors, patients, dentists and pharmacists alike.įor doctors, it replaces the need for handwritten documents, which can be tedious and time-consuming. Explore free apps to determine which can serve your practice best before you make a commitment. ![]() Some e-prescription services are entirely free, while others rely on subscriptions or one-time purchases. Some services boast cross-platform compatibility, meaning you can access the software through various devices. It typically comes in the form of an online application accessible through the internet. E-prescribing software allows physicians and other licensed medical professionals to create electronic prescriptions for patients. ![]() Of the remaining three, I'd have to crown Hangouts the king. The biggest differentiating factor between these apps was the ability to call any number for free, and this is where Skype and Viber lose out. Call any U.S.-based number for free: No (free to call Viber users)Īfter quite a bit of testing, it became clear that call quality was pretty much the same across all five of the aforementioned services.If you've set up a Google Voice account in the past, you'll get the added benefits of free SMS messaging as well as a dedicated phone number that can be forwarded to other lines and devices. This enables you to call any number in the United States for free-even from your computer. To use Hangouts as a VoIP service, though, you'll have to install an additional app called Hangouts Dialer. This means with the base Hangouts app (which comes pre-installed on most Android phones), you can send messages and video chat with anyone who has Hangouts installed on one of their devices. At its core, Hangouts is a hybrid of the old Google Chat and the soon-to-be-retired Google Voice. Google has had many different communications platforms throughout the years, and they've recently combined several of these into an all-encompassing service called Hangouts. Cross-platform support: Linux, Windows, Mac OS X, Android, iOS.Outbound phone number: Assigned personal number (with purchase).Call any U.S.-based number for free: No (free to call Skype users).Recently, Vonage has even added IP-based text messaging and a video calling feature, but both of these features require that all parties have the Vonage app installed. If both parties have the Vonage app installed, you'll be able to make free calls to anywhere in the world. They've kept themselves near the top of the heap with a mobile app that offers free calling within the United States, as well as a few other nice features. Option 1: VonageĪs one of the pioneers of VoIP-based landline calling in the early 2000s, Vonage is a stalwart in this market even today. The apps are listed in no particular order, but I'll offer my opinion on which is the best option at the end of this article. resident, so the information provided below may vary depending on your country. This comparison was done from the perspective of a U.S. Some offer free calling within the United States to any U.S.-based phone number, while others will require that the party you're calling also has the same app installed on their device if you want to go the free route. Its audio quality during calls is pretty good by general standards.All five of these apps offer free VoIP calling in one way or another. Viber is a good alternative in the wide range of instant messaging apps for Android. Thanks to this, you can continue your conversations on your computer, making and receiving calls no matter where you are. ![]() It's better if this connection is WiFi or 3G, since using this app with a data plan can be more expensive than using the phone.Ī positive element is that besides the mobile app, it has a client for Windows and Mac. Once you install the app on your device, all you have to do is let it access your contact list, and in a matter of seconds you can start calling with pretty good audio quality and no expenses except the Internet service. In fact, within the app, you can find a sticker store where you can find new tags to buy and use in conversations. Thanks to Viber, you can share text messages, photographs, messages with videos or voice, locations, emoticons, and tags. With it, you can send text messages and make phone calls for free, so long as you have an Internet connection. Viber, which was originally called Viber - Free Phone Calls, is an application which is similar to Skype. ![]() This is one of the reasons it’s important to look at larger samples and test them whenever possible. They appear darker here because they’re against a white backdrop. Keep in mind that these will still look almost white on the walls. Cool and Warm White Paintsīelow is are examples of cool and warm white paints. Whiter, lighter colors have high LRV’s since they reflect more light, and darker colors have low LRV’s reflect less light and absorb more of it. ![]() The scale ranges from 0 (absorbs all light) to 100 (absorbs no light or reflects 100%). LRV, or light reflective value, is a number that’s assigned to paint colors to indicate the percentage of light that reflects off of them, or basically how light they appear on a wall. It’s still as popular as it was that year, and makes a great choice for a warm, creamy white for walls. A highly popular shade of white, Simply White was actually Benjamin Moore’s Color of The Year back in 2016. More drops and more tints will affect the depth of color and the undertones. It balances out really well for both lighting conditions to create the perfect neutral backdrop. You can also buy Benjamin Moore Paint Color Samples (8 oz.), 4x8 swatches or peel & stick samples online. You can buy color samples from your local Benjamin Moore retailer. It can fit across scenarios and mixes white and gray undertones. A drop of black, blue, or green will produce a cool undertones. 'Try On' Our Most Popular Colors Sampling one or more of our most popular paint colors is the best way to find a paint color you’ll love for years to come. The Manchester Tan by Benjamin Moore falls in the category of chameleon colors. Deeper greige paint colors, like Pashmina AF-100 and Silver Fox 2108-50, offer a balance between light and dark while still providing flexibility for furniture, lighting. Variations of tints are unlimited and will determine the undertones of your paint.įor example, a drop of yellow oxide or red oxide will give your white paint a warm undertone. Pastels with warm undertones, like First Light 2102-70, a former Benjamin Moore Color of the Year, and Windham Cream HC-6, flatter like a beige without reading too neutral. There are 16 different colors in the Benjamin Moore paint tinting machines. But don’t worry, these formulas have been used for decades and are always spot on. You’d be surprised at the colors the make up your white and neutral paints. I’m glad you asked! Did you know that unless a color is premixed at the paint factory, it has at least a few drops of colorant added to it at the paint store? I worked at our family paint shop for 30+ years and can attest to this. Cooler whites have green, blue, and violet undertones to them. ![]() Warmer whites have red, yellow, and orange tints undertones. Most every white has some type of undertone to it. What’s the difference between a warm white and a cool white? They have the fewest undertones and therefore appear whiter than others. The whitest paints from Benjamin Moore are Chantilly Lace and Super White. Which Benjamin Moore white paint is the whitest? Best white and off-white paint colors from Benjamin Moore.What are the most popular whites from Benjamin Moore?.What's the difference between a warm white and a cool white?.Which Benjamin Moore white paint is the whitest?.Best Benjamin Moore White and Off-White paint colors. ![]() Book of 112 pages comes with 36-week progressive program along with scope, sequence charts and answer keys. As usual, there will be pre-sales for season members and subscribers to the Rays Insider email newsletter who are signed up by Sunday. Evan-Moor Daily Math Practice Book for grade 1 helps to keep skills sharp with focused practice presented in standardized testing formats. During the week I have swimming practice on Mondays, I do taekwondo on Tuesdays and tennis on Thursdays. Having clinched their fifth straight playoff berth Sunday, the Rays will put tickets for potential American League Wild Card Series and Division Series home games on sale Sept. To make room, the Rays optioned infielder Tristan Gray, who had an impressive two-game first big-league stint, and veteran righty reliever Chris Devenski, who had a 4.15 ERA over six games since being signed Aug. ![]() Time: 45-75 minutes Purpose: (1) Differentiate instruction for all learners and (2) provide ongoing practice and review of previously taught skills There are 2 different things going on during this portion of your math block. More than 2,550 addition, subtraction, multiplication, division, fractions, and decimals assignments will help your kid to master these necessary skills to.US8. Shortstop Taylor Walls was activated from the paternity list after the Sunday birth of a son, and lefty reliever Jalen Beeks was called up Monday. Math Centers and Teacher Led Small Group. The team record for steals is 194 in 2009. They also have a chance to break the franchise home run record of 228, set in 2017. Daily Math Practice provides a weeks worth of problems ideal for morning work, homework, or review. The Rays lead the AL in steals, 155-152 over the Royals, and have a chance to become the first team since the 1995 Indians to lead the league in both categories. Arrives by Thu, Sep 21 Buy Kindergarten Math Workbook with Dinosaurs (Addition & Subtraction): Daily Math Practice Workbook - Daily Basic Math Practice for. ![]() Number talks focus on mental math and computation strategies, so if your students need more meaningful math fact practice or struggle to use efficient strategies, number talks is the way to go. The Rays go into play Tuesday tied with the Angels - who they host - for the American League lead with 216 homers the Twins have 215. I talk about number talks quite often, because I think this is a wonderful way to start your math time. If Raley and Josh Lowe get to 20, the septuple would match the second most in major-league history (the 2019 Twins had eight). ![]() What Needs to Go Right: Well, Dak Prescott of course. The Cowboys have to resolve some burning questions at quarterback. Battle Lines: Still Questions to Answer at QB - Kurt Daniels, Dallas Cowboys fans waiting to find out if Cooks can still cover 40 yards in 4.33 seconds, as he did during the 2014 NFL draft combine, shouldn’t hold their breath for a re-race. He’ll look to get back to those numbers when catching throws from Dak Prescott in 2023. Cooks has topped 1,000 receiving yards in a season six times, most recently in 2021. The Cowboys acquired Cooks from the Houston Texans in March. Make sure you do what you’re supposed to do so you can stay in league as long as you possibly can,’” Gallup said. Wide receiver Michael Gallup added that Cooks, who is entering his 10th NFL season, has been focused on helping his teammates ensure they will be able to enjoy similarly long careers.“He tells everybody, ‘When you are you’re done with this, you’re done with that, make sure you stretch. I love each and every part of it,” Lamb said. “He has a great understanding of routes and time clocks, and specifics of getting in and out of breaks.”įellow receiver CeeDee Lamb credited Cooks with teaching him new tricks of the trade as the two ran routes during the Cowboys’ recent minicamp.“I’m a visual learner and I’m just watching him as he runs his routes, the way he preps, the way he practices, how he goes about his business. Head coach Mike McCarthy said Cooks boasts other skills that build on his speed, ESPN’s Todd Archer reported.“I think he’s an exceptional route-runner,” McCarthy said. ![]() Newcomer Brandin Cooks has been making an impression on his new teammates. Cowboys’ Brandin Cooks Draws Rave Reviews from CeeDee Lamb, Mike McCarthy, More - Julia Stumbaugh, Bleacher Report His route running may still be a work in progress, but he was spotted with Dak and some of the guys getting some work earlier this year. This coaching staff should utilize his athleticism and quickness off the line. At this point, Turpin’s similarities to Beasley are much more than their sub-5’10” height. Michael Gallup and Brandin Cooks will round out the trio.Ĭooks’ deep speed offers match-up problems, but working in concert with Turpin’s shiftiness underneath could be deadly. CeeDee Lamb is undoubtedly the team’s certified alpha and number one receiver. Luckily for Turpin, a role is available that fits his physical profile and skill set better than any of the receivers ahead of him on the depth chart. He will walk in as a certified Pro Bowl specialist, but long-term success can be less secure on special teams. We are in a different year, and the start of his second season is quickly approaching.Įxpectations placed on himself may be higher than any attached to him by coaches and fans. Some of that was on him, and a portion of the responsibility fell at the feet of the play designer. Add insult to injury, Dallas decided to put him in a divisional round football game on the final play, only to find himself on the wrong end of a whooping stick. As a receiver, he was tossed two targets and hauled in 1 reception for the entire season. There was little to KaVontae Turpin’s receiving role last season. IS IT TIME FOR DALLAS TO CAPITALIZE ON KAVONTAE RECEIVING SKILLS? It may take a few games for Vaughn to find his way, but his speed, elusiveness, and sure hands should escalate the process. Hauling in 116 receptions for 1280 yards in his three seasons as a starter will earn you the status of a complete dual-threat prospect. The numbers he put up on the receiving end were more than respectable. That’s because his running ability is so fun to watch. That part of his game is clearly seen on tape.Īt Kansas State University, his receiving prowess gets criminally overlooked. Although he has no documented 10-yard split time, his explosive speed off the line of scrimmage enables him to create separation and get open consistently. The stop-on-a-dime moves, and ankle-breaking shiftiness make defenders look like low-level intramural athletes trying to find their skills of yesteryear. The sub-4.5 speed is more than suitable to get up the field. The Cowboys look for a way to get these two exciting playmakers on the field.ĭEUCE VAUGHN IS THE EASY ANSWER FOR A SAFETY BLANKETĬould there be a better underneath option than Deuce Vaughn? If there is, please educate me on who that is. Can Dallas a find a way to get this electrifying duo on the field - on offense? - Jermaine Arvie, Inside the Star Don’t be surprised if he finds the back of the net in his debut. Lionel Messi à propos du PSG : « Durant deux ans, je nétais pas heureux ». 29, we’ll be ready to see if he won’t waste any time in keeping his magical ways on the pitch going with PSG. Mitchell van Bergen almost scores a debut goal for Reims but he's. Messi is known for his amazing passes and out of this world goals.Ĭome Aug. Lionel Messi's debut ends in a 2-0 win for PSG - but it was Kylian Mbappe who was the matchwinner. ![]() With Messi now in the fold, PSG is ready to try and win the league title, while also making a push for the Champions League glory. Leo Messis best goals and assists for PSG - 2021/22.Les plus beaux buts et les plus belles passes décisives de Leo Messi pour le PSG - 2021/22.Leo Messi, no. ![]() The long-awaited debut of Lionel Messi in a PSG shirt finally happened as PSG beat Stade de Reims 2-0 in a Ligue 1 meeting to close out Matchday 4. How in the world can these three be stopped? Messi makes his PSG debut: Heres how it went. and Kylian Mbappe will likely be keeping their defenders up late at night. While countless hearts remain broken in Catalonia knowing that Messi is no longer playing for Barcelona, folks over in Spain are still wishing the best for him at his new team.Īs for Reims, the idea of having to stop an attack that will feature Messi, Neymar Jr. Messi’s first Ligue1 match has smashed the record and become the most viewed French league match in Spanish viewing history IbaiLlanos: 343.000 spectators and 2m unique Spanish viewers. 29 when the team takes on ReimsĪfter his sensational 17 years with Barcelona’s senior team, Messi will now start his next chapter with PSG. The club has scheduled a press conference which will kick off at 11am local time. Le premier match de Lionel Messi sous les couleurs du PSG se dessine un peu plus du côté de Reims, le dimanche 29 août, en clôture de la 4e journée de Ligue 1 /fR15OMdQQM- L'ÉQUIPE AugLionel Messi will make his Paris Saint-Germain debut on Aug. Messi is expected to be officially unveiled as a PSG player on Wednesday 11 August. Obviously, this is going to be a showdown that millions of fans across the world will be watching. The countdown is now less than a week away.Īccording to French outlet L’Equipe, Messi will make his debut for his new club on Aug. 29, the long wait will be over for Paris Saint-Germain fans who want to see Lionel Messi make his debut for the Ligue 1 team.Īs soon as it was announced that Lionel Messi had agreed to a two-year deal with Paris Saint-Germain, fans all over France have been counting down the days to see him in action. Lionel Messi has made his Paris Saint-Germain debut, coming on for Neymar after 64 minutes in a clash against Reims. It’s believed Messi will have his own protection team guarding himself and his family but the club expect his arrival to be manageable when he’s on club duty.Īnd he pointed to Messi’s last visit to the USA before the World Cup in Qatar as a successful security operation with Miami’s own security detail constantly updating their practices.Come Aug. Free-kick to PSG as Lionel Messi is fouled. Majority owner Jorge Mas has already held talks with the club’s protection department with the safety of the Argentinian star and his family a major priority.Īnd Mas has insisted an exhaustive list of extensive new measures will be implemented to ensure the safety of Messi as well as the rest of the club’s players when the team is on their travels and at their own Fort Lauderdale stadium and training facility. Lionel Messi's debut ends in a 2-0 win for PSG - but it was Kylian Mbappe who was the matchwinner. ![]() Cest désormais chose faite après sa sublime réalisation contre les Citizens. Et quel but LArgentin, qui a rejoint Paris cet été, navait pas encore eu le bonheur de communier avec le public parisien après un but. Lionel Messi's impending arrival in the MLS has brought excitement to fever-pitch level in Miami and across the game in America.īut it’s brought a big headache for Miami’s security team. Lionel Messi a inscrit son premier but avec le PSG mardi soir contre Manchester City en Ligue des champions. ![]() Sebastian Frej/Getty Images) Messi's impending arrival creates security headache ![]() If you’re one of the tens of millions of Americans who were immunized against Covid-19 at a pharmacy or grocery store participating in the retail pharmacy program, you may be able to access electronic version of your records through the brand’s website.įor example, if you received your shots at Walmart, you can access digital copies of your vaccination records through its branded website and app. Other states, including Indiana, Colorado and Georgia, can provide electronic copies of vaccination records in PDF form but not via a QR code. “However, these talks are preliminary and there is no definite timeline.” ![]() “There are currently discussions regarding adding a QR feature to the MyIR Mobile application,” said Shelby Anderson, a spokesperson for the Washington State Department of Health, via email. While it MyIRMobile does not provide a QR code, it does offer an official, verified record from each participating state. MyIRMobile is available in Arizona, District of Columbia, Louisiana, Maryland, Mississippi, North Dakota, Washington and West Virginia, and it is rolling out soon in Puerto Rico. New York has its Excelsior Pass, Hawaii has its Hawaii Safe Travels system and California has its own Digital Covid-19 Vaccine Record.Įight more states and territories use a platform called MyIRMobile, which allows residents to obtain their immunization records on a PDF that can be either printed or stored on their phones. These QR code is typically PIN-protected so nobody else can access your records. Every state’s health department keeps track of immunization records and can tell you whether it’s possible to get a digital proof of your Covid status. The first step is to find your state on the CDC’s list of state and regional immunization registries.Ī small handful of states have developed their own digital apps that can provide a QR code that verifies the vaccination status of residents. The best place to start is where you got vaccinated. And Americans, regardless of where they live, have more options than they may realize.Ĭheck with your state’s health department. ![]() With no federal digital solution in the United States, each state is left to decide whether and how it will provide residents with a way to access state-verified records in a digital format. ![]() still relying on paper vaccination records from the Centers for Disease Control and Prevention - an analog solution that is flimsy, easily faked, and requiring human scrutiny to confirm authenticity. Follow her adventures on Instagram.The Biden administration has declined to get behind a national app, and the topic of vaccine passports has become a political hot potato, with 15 Republican-leaning states limiting or banning vaccine passports via legislation or governors’ executive orders. When she's not in New York City, she likes to spend her time at the beach or exploring new destinations and hopes to visit every country in the world. While some states like Hawaii and New York have embraced the idea of vaccine passports, the concept has become a flashpoint, and other states - like Texas, Florida, and Arizona - have banned them.Īlison Fox is a contributing writer for Travel Leisure. "We've been working with a couple of other private sector partners about working to get access to the state vaccination records, and we believe that that would help them to get vaccination records in other states as well."Ĭurrently, travelers heading to Hawaii can skip quarantine if they get tested before traveling at a "trusted partner" site. The challenge is about verification - about vaccination done in other states," Gov. "We don't have a firm timeline on trans-Pacific. While the program will eventually be expanded to travelers from outside Hawaii, it must wait until the state can "verify vaccine information and can provide the same assurance of integrity on a nationwide level," according to the guidance. It's big," one traveler who was headed from Oahu to Maui told Hawaii News Now. "It's really helpful and I think it will help the islands to stay down where we should be. The concept is in line with the CDC's recommendation that fully vaccinated Americans can travel domestically without the need to get tested or quarantine. Kent Nishimura/Los Angeles Times via Getty Images ![]() Despite its functional importance and emergence as a mutational hotspot, the N protein has not been widely studied because of the absence of simple and safe cell-based assays. However, most mutations in circulating variants occur outside of the S gene and are thus inaccessible by this approach ( 8).Īll SARS-CoV-2 variants of interest or concern defined by the World Health Organization contain at least one mutation with >50% penetrance within a seven–amino acid span (199 to 205) in the nucleocapsid (N) protein, which is required for replication, RNA binding, packaging, stabilization, and release ( 8). Current studies employ spike (S) protein–pseudotyped lentivirus systems for evaluation of S-mediated ACE2 receptor binding and cell entry ( 6, 7). ![]() Furthermore, technical challenges impede efforts to generate mutant infectious clones of SARS-CoV-2 ( 1– 5). Understanding the molecular determinants of enhanced infectivity is central to vaccine and therapeutic development, but research is hindered because SARS-CoV-2 can be studied only in a biosafety level 3 (BSL-3) laboratory. The COVID-19 pandemic is a leading cause of death globally, owing to the ongoing emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with increased transmissibility. SC2-VLPs provide a platform for rapid testing of viral variants outside of a biosafety level 3 setting and demonstrate N mutations and particle assembly to be mechanisms that could explain the increased spread of variants, including B.1.617.2 (Delta, which contains the R203M mutation). In SC2-VLPs, four nucleocapsid (N) mutations found universally in more-transmissible variants independently increased messenger RNA delivery and expression ~10-fold, and in a reverse genetics model, the serine-202→arginine (S202R) and arginine-203→methionine (R203M) mutations each produced >50 times as much virus. In this study, we show that SARS-CoV-2 virus-like particles (SC2-VLPs) can package and deliver exogenous transcripts, enabling analysis of mutations within all structural proteins and at multiple steps in the viral life cycle. Doudna, Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editingĮfforts to determine why new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants demonstrate improved fitness have been limited to analyzing mutations in the spike (S) protein with the use of S-pseudotyped particles. Melanie Ott, Methodology, Project administration, Resources, Supervision, Validation, Hayashi, Investigation, Resources, Writing - review & editing, ![]() Khalid, Conceptualization, Investigation, Methodology, Resources, Supervision, Validation, , † Takako Tabata, Investigation, Methodology, Validation, Visualization, Writing - original draft, Writing - review & editing, # Taha, Conceptualization, Investigation, Methodology, Resources, Validation, Visualization, Writing - original draft, Writing - review & editing, # Syed, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing, # |
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